Molecular biology studies biological activity at the molecular level. Projects in the lab may include isolating DNA or RNA molecules from tissue for further analysis or sequencing. We use HPLC, mass spectroscopy and gel electrophoresis for the isolation of proteins and evaluating those proteins for modifications. Isolation or evaluating proteins for modifications can employ HPLC, mass spectroscopy or gel electrophoresis. Western blots and ELISAs are some of the more common techniques used to determine protein concentrations. In other experiments, we look to quantify and understand the effects of mitochondrial DNA heteroplasmy. With our hospital collaborators, we investigate cellular adherins, intra- and extra-cellular signaling, cell receptor signaling pathways, and mechanisms for apoptosis. In the past, we have identified and quantitated a variety of molecular markers from blood and interstitial fluid.
Dimerization of receptors involved with breast cancer.
Image is the courtesy of the National Cancer Institute.