| Specimen | CYTOGENETIC STUDIES OF PRODUCTS OF CONCEPTION
(KARYOTYPE ANALYSIS)
- - - - PROCEDURES FOR PHYSICIANS - - - -
1. SPECIMEN COLLECTION: (from stillborn or
liveborn with dysmorphic features)
a) AMNIOTIC FLUID: 15 ml in sterile plastic
50 ml conical tubeadditional 5 ml is
needed by OB/GYN Lab to perform
alphafetoprotein testing.
b) BLOOD: sodium heparin tube (green top, no
gel).
c) TISSUES AND POC: Sterile chromosome
transport media.<<< DO NOT USE FORMALIN OR
ALCOHOL>>>.
d) If infant is alive, draw blood in a green-
top tube(2 to 3 ML is sufficient).
e) PUBS: Sodium heparin tube (green top, no
gel). Send stat; do not refrigerate.
f) BONE MARROW: sodiumheparin tube ( green
top, no gel).
2. SPECIMEN LABEL: Labels must include type of
specimen (peripheral blood, cord blood,
placenta, etc.) and date and time of
collection.
3. SPECIMEN STORAGE (evenings & weekends):
Products of conception(POC) must be stored in
tissue culture media (NOT SALINE). This is
supplied by the send-out area (410-601-
4973).All samples can be refrigerated in
sterile containers.Specimens are viable for
72 hours. Transport in the morning to
Pathology Central Processing.
4. TEST REQUESTING INFORMATION: Fill out
Chromosome Study Request Form. Note all
abnormalities, pertinent clinical history,
insurance carrier, and physician name and
phone number. This form should be available
in the Labor/Delivery Room, Nursery, and
Pathology Send-out area. |
| Processing | 1. Make sure specimen is properly labeled with:
- patient name and I.D. Number
- type of specimen (cord blood,
peripheral blood,amniotic fluid,
tissue, etc.
- date and time of collection
2. Check that the container is correct:
- bone marrow, peripheral or cord blood:
green-top tube
- amniocentesis fluid: plastic tube
- placental tissue: sterile urine cup
with sterile transport medium
3. Be sure that the form "CHROMOSOME STUDY
REQUEST FORM" accompanies all specimens.
4. If there are any problems with #1-3 above, call
the house officer
or SendOuts area (4973) immediately.
5. Test request using the test code KARYO. Use
the proper
specimen code:
B Blood (default specimen)
AM amniotic fluid
BM bone marrow
TS tissue
6. If specimen is received at night or weekends,
DO NOT SPIN--
just refrigerate. DO NOT FREEZE.
7. On day shift (weekdays) notify Send-out Area. |
